Master Mix Ready To Load, 100µL (25×20µL PCR rxns)

It is an optimised, pre-mixed formulation containing all essential components for successful DNA amplification - including DNA Polymerase, reaction buffer, MgCl₂, dNTPs, and a tracking dye for direct loading onto agarose gels.

Convenience & Time-Saving

Designed for ease of use, this master mix reduces hands-on preparation time and minimises the risk of contamination or pipetting errors. The inclusion of gel tracking dyes eliminates the need to add loading buffer post-PCR, enabling samples to be loaded directly onto an agarose gel for electrophoresis.

Robust Performance

The formulation ensures high yield and reproducibility across various DNA templates and amplicon sizes. Its robust enzymatic activity is suited for standard and routine PCR applications, delivering consistent results even with low-copy targets or complex DNA mixtures.

Storage & Stability

This Master Mix Ready to Load should be stored at –20°C for long-term use. Thawed mixes can be stored at 4°C for up to one month without significant performance loss.

Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.

Applications

Ideal for general molecular biology applications, including DNA cloning, genotyping, screening, and sequencing workflows.

The mix is especially suitable for laboratories seeking a fast, reliable PCR setup with minimal additional steps.

Product Features

• Ready-to-Load Format: Enables direct gel electrophoresis post-PCR, saving time and reducing errors.
• Optimised 5× Concentration: Ensures robust amplification with minimal preparation.
• High Reproducibility: Supports consistent results across various sample types and conditions.
• Integrated Tracking Dye: Eliminates the need for a separate gel loading buffer.
• All-in-One Formulation: Includes buffer, dNTPs, MgCl₂, and enzyme - no additional components required.
• Cold Storage Compatible: Maintains stability at 4 °C for up to one month once thawed.
• Versatile Use: Suitable for a broad range of standard PCR applications.